365 papers
This study introduces a new stable polygenic mouse model of depression (H-FST) that, unlike its predecessor (H-TST), exhibits distinct behavioral and molecular profiles with opposite antidepressant responses, thereby enabling the identification of human biomarkers specific to clinical subgroups of treatment responders.
This study characterizes *Toxoplasma gondii* strains causing ovine abortion in Uruguay, revealing extensive non-clonal diversity with multiple evolutionary origins, links to human infections, and virulence factors like ROP5 copy number variation that underscore the need for enhanced genomic surveillance in South America.
This paper presents a non-invasive protocol for determining the sex of various coleoid cephalopod species as early as three hours post-hatching by utilizing skin swabs and quantitative PCR.
This study reveals that in metazoans, the DNA helicase activation required for chromosome replication relies on the cooperative function of MCM10 and SLD-2/RECQL4, a mechanism whose disruption leads to synthetic lethality and is linked to human disease syndromes.
This study reveals that MCM10 and RECQL4 function cooperatively and redundantly through direct interaction and ssDNA binding to activate the CMG helicase during human DNA replication initiation, with RECQL4 serving as the primary factor and MCM10 acting as a critical backup.
This study reveals that the splicing factor SRSF1 regulates polyadenylation site selection and RNA isoform determination through a dual mechanism involving direct RNA binding independent of U1 snRNP and coordinated interactions with U1 snRNP and RNA Polymerase II to modulate transcription elongation.
This study identifies TXNDC15 as a critical regulator of endoplasmic reticulum protein quality control that fine-tunes the E3-ubiquitin ligase MARCHF6 to selectively degrade excess membrane protein subunits while protecting ciliary proteins, thereby ensuring proper ciliogenesis and preventing Meckel-Gruber syndrome.
This study reveals that the C-terminus of the RNA splicing factor Prp45 (SKIP) plays an evolutionarily conserved, extra-spliceosomal role in stabilizing the scaffold protein Lge1 to facilitate histone H2B ubiquitination and maintain proper cellular morphology.
This study reveals that a specific subset of maize protein storage genes, typically silenced by TE-like heterochromatin in most tissues, undergoes maternal demethylation in the endosperm to switch to a euchromatic state, enabling exceptionally high and often imprinted expression.
In a Drosophila tauopathy model, pathological tau induces promoter-proximal RNA polymerase II stalling, which specifically disrupts the elongation of long neuronal genes and leads to their downregulation.
This study identifies DNA damage response proteins, particularly the helicase-like transcription factor HLTF, as key host factors that drive the formation of large internal deletions in HIV-1 proviruses, revealing a previously undescribed cellular defense mechanism that generates defective viral genomes.
This study demonstrates that pirfenidone attenuates renal fibrosis and improves kidney function in autosomal dominant polycystic kidney disease by targeting myofibroblast activation and extracellular matrix production, offering a promising complementary therapeutic strategy despite not reducing cyst burden.
This study reveals that 3' nucleotide asymmetry acts as a conserved, previously unrecognized mechanism that cooperates with 5' features to direct accurate miRNA strand selection and loading into the miRISC complex in both *C. elegans* and human cells.
This study reveals that BRCA1 regulates RAD51 to confer a dependency on SCAI and REV3 for maintaining stalled replication fork integrity and preventing DNA breakage, a process distinct from BRCA1's canonical fork protection role and independent of resection factor binding.
This study characterizes the miRNA cargo of ovine granulosa cells and their derived extracellular vesicles following exposure to bisphenols A and S, revealing that while BPA and BPS have limited effects on cellular miRNA expression, they significantly alter the miRNA profiles of secreted vesicles, including the downregulation of miR-1306 homologs.
The paper introduces MS Andrea, a novel open modification search engine that directly identifies and scores combinations of up to four variable modifications per peptide at the PSM level without predefined lists, achieving superior identification rates and enhanced interpretability compared to existing tools like MSFragger and Sage.
This study demonstrates that 3D printing polypropylene directly onto chromatography paper creates high-integrity, high-resolution hydrophobic barriers for capillary-driven microfluidic devices, successfully validated through a fluorescence-based biosensing assay.
This study evaluates a tiling PCR primer scheme adapted for Oxford Nanopore sequencing of Hepatitis B virus, finding that while the method is feasible, uneven amplification efficiency across amplicons and strong dependence on viral load (Ct values) limit consistent full-genome recovery, necessitating further optimization of the primer design.
This study demonstrates that long-term pentosan polysulfate (PPS) exposure induces retinal pigment epithelium injury by impairing mitochondrial respiration, disrupting protective mechanisms against oxidative stress, and causing structural and functional deficits including reduced phagocytosis and increased cell death.
This study reveals that neural tissue vitrification induces selective transcriptomic vulnerability specifically affecting membrane-integrated and secretory pathway proteins, with the hippocampus showing greater sensitivity than the cortex, highlighting the need for transcript-level evaluation to optimize cryopreservation protocols.